Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.
Identifieur interne : 000124 ( Main/Exploration ); précédent : 000123; suivant : 000125Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.
Auteurs : Birgit Kersten [Allemagne] ; Ana Paula Leite Montalvão [Allemagne] ; Hans Hoenicka [Allemagne] ; Cristina Vettori [Italie] ; Donatella Paffetti [Italie] ; Matthias Fladung [Allemagne]Source :
- Transgenic research [ 1573-9368 ] ; 2020.
Abstract
Next-generation sequencing (NGS) approaches are attractive alternatives to the PCR-based characterisation of genetically modified plants for safety assessment and labelling since NGS is highly sensitive to the detection of T-DNA inserts as well as vector backbone sequences in transgenic plants. In this study, two independent transgenic male Populus tremula lines, T193-2 and T195-1, both carrying the FLOWERING LOCUS T gene from Arabidopsis thaliana under control of a heat-inducible promoter (pHSP::AtFT) and the non-transgenic control clone W52, were further characterised by NGS and third-generation sequencing. The results support previous findings that the T-DNA was hemizygously inserted in one genomic locus of each line. However, the T-DNA insertions consist of conglomerations of one or two T-DNA copies together with a small T-DNA fragment without AtFT parts. Based on NGS data, no additional T-DNA splinters or vector backbone sequences could be identified in the genome of the two transgenic lines. Seedlings derived from crosses between the pHSP::AtFT transgenic male parents and female wild type plants are therefore expected to be T-DNA splinter or vector backbone free. Thus, PCR analyses amplifying a partial T-DNA fragment with AtFT-specific primers are sufficient to determine whether the seedlings are transgenic or not. An analysis of 72 second generation-seedlings clearly showed that about 50% of them still reveal the presence of the T-DNA, confirming data already published. To prove if unanticipated genomic changes were induced by T-DNA integration, extended future studies using long-range sequencing technologies are required once a suitable chromosome-level P. tremula reference genome sequence is available.
DOI: 10.1007/s11248-020-00203-0
PubMed: 32356192
PubMed Central: PMC7283205
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.</title><author><name sortKey="Kersten, Birgit" sort="Kersten, Birgit" uniqKey="Kersten B" first="Birgit" last="Kersten">Birgit Kersten</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. birgit.kersten@thuenen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Leite Montalvao, Ana Paula" sort="Leite Montalvao, Ana Paula" uniqKey="Leite Montalvao A" first="Ana Paula" last="Leite Montalvão">Ana Paula Leite Montalvão</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Hoenicka, Hans" sort="Hoenicka, Hans" uniqKey="Hoenicka H" first="Hans" last="Hoenicka">Hans Hoenicka</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Vettori, Cristina" sort="Vettori, Cristina" uniqKey="Vettori C" first="Cristina" last="Vettori">Cristina Vettori</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI</wicri:regionArea><wicri:noRegion>FI</wicri:noRegion></affiliation></author><author><name sortKey="Paffetti, Donatella" sort="Paffetti, Donatella" uniqKey="Paffetti D" first="Donatella" last="Paffetti">Donatella Paffetti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Food, Environment and Forestry, Agricultural Genetics Section, University of Florence, P. le delle Cascine 18, 50144, Florence, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agriculture, Food, Environment and Forestry, Agricultural Genetics Section, University of Florence, P. le delle Cascine 18, 50144, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. matthias.fladung@thuenen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32356192</idno><idno type="pmid">32356192</idno><idno type="doi">10.1007/s11248-020-00203-0</idno><idno type="pmc">PMC7283205</idno><idno type="wicri:Area/Main/Corpus">000323</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000323</idno><idno type="wicri:Area/Main/Curation">000323</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000323</idno><idno type="wicri:Area/Main/Exploration">000323</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.</title><author><name sortKey="Kersten, Birgit" sort="Kersten, Birgit" uniqKey="Kersten B" first="Birgit" last="Kersten">Birgit Kersten</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. birgit.kersten@thuenen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Leite Montalvao, Ana Paula" sort="Leite Montalvao, Ana Paula" uniqKey="Leite Montalvao A" first="Ana Paula" last="Leite Montalvão">Ana Paula Leite Montalvão</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Hoenicka, Hans" sort="Hoenicka, Hans" uniqKey="Hoenicka H" first="Hans" last="Hoenicka">Hans Hoenicka</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Vettori, Cristina" sort="Vettori, Cristina" uniqKey="Vettori C" first="Cristina" last="Vettori">Cristina Vettori</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI</wicri:regionArea><wicri:noRegion>FI</wicri:noRegion></affiliation></author><author><name sortKey="Paffetti, Donatella" sort="Paffetti, Donatella" uniqKey="Paffetti D" first="Donatella" last="Paffetti">Donatella Paffetti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Food, Environment and Forestry, Agricultural Genetics Section, University of Florence, P. le delle Cascine 18, 50144, Florence, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agriculture, Food, Environment and Forestry, Agricultural Genetics Section, University of Florence, P. le delle Cascine 18, 50144, Florence</wicri:regionArea><wicri:noRegion>Florence</wicri:noRegion></affiliation></author><author><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. matthias.fladung@thuenen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, 22927, Grosshansdorf</wicri:regionArea><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>22927, Grosshansdorf</wicri:noRegion><wicri:noRegion>Grosshansdorf</wicri:noRegion></affiliation></author></analytic><series><title level="j">Transgenic research</title><idno type="eISSN">1573-9368</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Next-generation sequencing (NGS) approaches are attractive alternatives to the PCR-based characterisation of genetically modified plants for safety assessment and labelling since NGS is highly sensitive to the detection of T-DNA inserts as well as vector backbone sequences in transgenic plants. In this study, two independent transgenic male Populus tremula lines, T193-2 and T195-1, both carrying the FLOWERING LOCUS T gene from Arabidopsis thaliana under control of a heat-inducible promoter (pHSP::AtFT) and the non-transgenic control clone W52, were further characterised by NGS and third-generation sequencing. The results support previous findings that the T-DNA was hemizygously inserted in one genomic locus of each line. However, the T-DNA insertions consist of conglomerations of one or two T-DNA copies together with a small T-DNA fragment without AtFT parts. Based on NGS data, no additional T-DNA splinters or vector backbone sequences could be identified in the genome of the two transgenic lines. Seedlings derived from crosses between the pHSP::AtFT transgenic male parents and female wild type plants are therefore expected to be T-DNA splinter or vector backbone free. Thus, PCR analyses amplifying a partial T-DNA fragment with AtFT-specific primers are sufficient to determine whether the seedlings are transgenic or not. An analysis of 72 second generation-seedlings clearly showed that about 50% of them still reveal the presence of the T-DNA, confirming data already published. To prove if unanticipated genomic changes were induced by T-DNA integration, extended future studies using long-range sequencing technologies are required once a suitable chromosome-level P. tremula reference genome sequence is available.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32356192</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-9368</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>06</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.</ArticleTitle><Pagination><MedlinePgn>321-337</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11248-020-00203-0</ELocationID><Abstract><AbstractText>Next-generation sequencing (NGS) approaches are attractive alternatives to the PCR-based characterisation of genetically modified plants for safety assessment and labelling since NGS is highly sensitive to the detection of T-DNA inserts as well as vector backbone sequences in transgenic plants. In this study, two independent transgenic male Populus tremula lines, T193-2 and T195-1, both carrying the FLOWERING LOCUS T gene from Arabidopsis thaliana under control of a heat-inducible promoter (pHSP::AtFT) and the non-transgenic control clone W52, were further characterised by NGS and third-generation sequencing. The results support previous findings that the T-DNA was hemizygously inserted in one genomic locus of each line. However, the T-DNA insertions consist of conglomerations of one or two T-DNA copies together with a small T-DNA fragment without AtFT parts. Based on NGS data, no additional T-DNA splinters or vector backbone sequences could be identified in the genome of the two transgenic lines. Seedlings derived from crosses between the pHSP::AtFT transgenic male parents and female wild type plants are therefore expected to be T-DNA splinter or vector backbone free. Thus, PCR analyses amplifying a partial T-DNA fragment with AtFT-specific primers are sufficient to determine whether the seedlings are transgenic or not. An analysis of 72 second generation-seedlings clearly showed that about 50% of them still reveal the presence of the T-DNA, confirming data already published. To prove if unanticipated genomic changes were induced by T-DNA integration, extended future studies using long-range sequencing technologies are required once a suitable chromosome-level P. tremula reference genome sequence is available.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kersten</LastName><ForeName>Birgit</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0001-9900-9133</Identifier><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. birgit.kersten@thuenen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leite Montalvão</LastName><ForeName>Ana Paula</ForeName><Initials>AP</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoenicka</LastName><ForeName>Hans</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vettori</LastName><ForeName>Cristina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paffetti</LastName><ForeName>Donatella</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Agriculture, Food, Environment and Forestry, Agricultural Genetics Section, University of Florence, P. le delle Cascine 18, 50144, Florence, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fladung</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0001-9301-8581</Identifier><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, 22927, Grosshansdorf, Germany. matthias.fladung@thuenen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>FL 263/21-1</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country>International</Country></Grant><Grant><GrantID>406678375</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country>International</Country></Grant><Grant><GrantID>LIFE08 NAT/IT/342 DEMETRA</GrantID><Agency>LIFE programme</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Transgenic Res</MedlineTA><NlmUniqueID>9209120</NlmUniqueID><ISSNLinking>0962-8819</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Biosafety research</Keyword><Keyword MajorTopicYN="Y">Early-flowering</Keyword><Keyword MajorTopicYN="Y">Poplar breeding</Keyword><Keyword MajorTopicYN="Y">Populus</Keyword><Keyword MajorTopicYN="Y">Transgene-free</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32356192</ArticleId><ArticleId IdType="doi">10.1007/s11248-020-00203-0</ArticleId><ArticleId IdType="pii">10.1007/s11248-020-00203-0</ArticleId><ArticleId IdType="pmc">PMC7283205</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biotechnol Adv. 2017 Mar - Apr;35(2):302-309</Citation><ArticleIdList><ArticleId IdType="pubmed">28131814</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12106-10</Citation><ArticleIdList><ArticleId IdType="pubmed">9770447</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2017 Nov 27;51:195-217</Citation><ArticleIdList><ArticleId IdType="pubmed">28853920</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Bioanal Chem. 2016 Jul;408(17):4595-614</Citation><ArticleIdList><ArticleId IdType="pubmed">27100228</ArticleId></ArticleIdList></Reference><Reference><Citation>Genomics Inform. 2015 Sep;13(3):81-5</Citation><ArticleIdList><ArticleId IdType="pubmed">26523132</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2005 Sep 9;309(5741):1694-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16099949</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2019 Jan 18;15(1):e1007819</Citation><ArticleIdList><ArticleId IdType="pubmed">30657772</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2003 May;52(1):161-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12825697</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2016 Jul 12;7:1009</Citation><ArticleIdList><ArticleId IdType="pubmed">27462336</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2019 Apr 29;70:699-726</Citation><ArticleIdList><ArticleId IdType="pubmed">30822113</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(12):e27310</Citation><ArticleIdList><ArticleId IdType="pubmed">22194782</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):E10970-E10978</Citation><ArticleIdList><ArticleId IdType="pubmed">30373829</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 Nov 1;25(21):2839-40</Citation><ArticleIdList><ArticleId IdType="pubmed">19737799</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO Rep. 2002 Dec;3(12):1152-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12446565</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Genet Eng Rev. 2006;23:209-37</Citation><ArticleIdList><ArticleId IdType="pubmed">22530509</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2014 Oct;12(8):1066-74</Citation><ArticleIdList><ArticleId IdType="pubmed">24975279</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2018 Oct 18;13(10):e0206085</Citation><ArticleIdList><ArticleId IdType="pubmed">30335843</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2019 Mar 12;10:236</Citation><ArticleIdList><ArticleId IdType="pubmed">30930911</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2017 Mar;36(3):493-504</Citation><ArticleIdList><ArticleId IdType="pubmed">28155116</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2001 Sep;213(5):731-40</Citation><ArticleIdList><ArticleId IdType="pubmed">11678277</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1997 May;11(5):945-57</Citation><ArticleIdList><ArticleId IdType="pubmed">9193068</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Oct 15;30(20):4556-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12384603</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2012 Dec;21(6):1255-64</Citation><ArticleIdList><ArticleId IdType="pubmed">22430369</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechniques. 2014 Feb 01;56(2):61-4, 66, 68, passim</Citation><ArticleIdList><ArticleId IdType="pubmed">24502796</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2016 May;36(5):667-77</Citation><ArticleIdList><ArticleId IdType="pubmed">27052434</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3640-5</Citation><ArticleIdList><ArticleId IdType="pubmed">18303117</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 Mar;49(5):947-59</Citation><ArticleIdList><ArticleId IdType="pubmed">17253985</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2015 Dec;208(4):1149-56</Citation><ArticleIdList><ArticleId IdType="pubmed">26192091</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2002 Aug;31(4):543-51</Citation><ArticleIdList><ArticleId IdType="pubmed">12182710</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6198-203</Citation><ArticleIdList><ArticleId IdType="pubmed">20308540</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2017 May;27(5):722-736</Citation><ArticleIdList><ArticleId IdType="pubmed">28298431</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2012 Jan 09;30(1):37-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22231091</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2006 Jul;4(4):369-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17177803</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2016 Apr;21(4):283-285</Citation><ArticleIdList><ArticleId IdType="pubmed">26897457</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Biotechnol. 2016 May 12;16(1):41</Citation><ArticleIdList><ArticleId IdType="pubmed">27176220</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol Resour. 2019 Jan;19(1):77-89</Citation><ArticleIdList><ArticleId IdType="pubmed">30118581</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2013 Oct 03;3:2839</Citation><ArticleIdList><ArticleId IdType="pubmed">24088728</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2008 Apr;6(3):213-25</Citation><ArticleIdList><ArticleId IdType="pubmed">18028290</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1999 Jan;260(6):574-81</Citation><ArticleIdList><ArticleId IdType="pubmed">9928937</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Mol Biol Rev. 2003 Mar;67(1):16-37, table of contents</Citation><ArticleIdList><ArticleId IdType="pubmed">12626681</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2005 Jul;138(3):1690-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15951487</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Italie</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Kersten, Birgit" sort="Kersten, Birgit" uniqKey="Kersten B" first="Birgit" last="Kersten">Birgit Kersten</name></noRegion><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><name sortKey="Hoenicka, Hans" sort="Hoenicka, Hans" uniqKey="Hoenicka H" first="Hans" last="Hoenicka">Hans Hoenicka</name><name sortKey="Leite Montalvao, Ana Paula" sort="Leite Montalvao, Ana Paula" uniqKey="Leite Montalvao A" first="Ana Paula" last="Leite Montalvão">Ana Paula Leite Montalvão</name></country><country name="Italie"><noRegion><name sortKey="Vettori, Cristina" sort="Vettori, Cristina" uniqKey="Vettori C" first="Cristina" last="Vettori">Cristina Vettori</name></noRegion><name sortKey="Paffetti, Donatella" sort="Paffetti, Donatella" uniqKey="Paffetti D" first="Donatella" last="Paffetti">Donatella Paffetti</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000124 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000124 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32356192
|texte= Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32356192" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |